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KMID : 1094720100150010110
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Biotechnology and Bioprocess Engineering
2010 Volume.15 No. 1 p.110 ~ p.118
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Effect of Reactor Surface on Production of Bacterial Cellulose and Water Soluble Oligosaccharides by Gluconacetobacter hansenii PJK
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Nasrullah Shah
Ha Jung-Hwa
Park Joong-Kon
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Abstract
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The effect of agar plates on the bacterial cellulose (BC) production in a static culture was investigated in order to find the role of agar component as a surface modifying agent. Two types of surface modified reactors (SMRs: SMRD and SMRB) were prepared by coating the bottom of the reactors with agar dissolved in distilled water and basal medium, respectively. The SMRs were used for BC and water soluble oligosaccharides (WSOS) production. Control was done by the same procedure using reactors without agar plate. In both types of SMRs, the maximum production rate was observed after the second day of cultivation compared to third day of cultivation in the case of the control. The maximum productions of BC 5.308 and 5.472 g/L were observed at the first batch using SMRs prepared with agar dissolved in distilled water (SMRDs) and SMRs prepared with agar dissolved in a basal medium (SMRBs), respectively. Similarly, in the daily-culture and successive batch strategy experiments the maximum amount of WSOS produced in the SMRs was almost double that of the control. The highest water holding capacity value 92.21 g/g was observed for BC formed in the SMRs prepared with 3.0% of agar. FTIR and XRD analyses were carried out to study the structural features of the prepared BC.
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KEYWORD
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bacterial cellulose, water soluble oligosaccharides, agar plates, production and productivity
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